Product Description

Delivery Time:In stock
Certification:ISO9001

PIPES Biological Buffers Piperazine-1 , 4-bis ethanesulfonic acid CAS NO 5625-37-6 99%

Quick detail:

Name	PIPES	Appearance	White crystalline powder
Assay,%	99.0-101.0	Loss on drying,%	≤0.5
MP/BP	≥300 °C	Solubility(5% 1 N NaOH.)	Clear, colorless solution
Ultraviolet absorbance/290nm(20% 1 N NaOH)	≤0.1	Heavy metals, ppm	≤5

1. Applications:

A. Biological Buffer.

B. MethA test kit for detection of high-density lipoprotein cholesterol for detecting HPV-E6 protein

C. Control of pH in aqueous urea-containing solutions utilizing amino acid-containing compositions

2. Location:

Biology/biochemistry laboratory, pharmaceutical factory, Bio-Research Facility , Hospital

Description:

> Formula:C8H18N2O6S2

> Weight: 302.36

> MP/BP :≥300 °C

> Storage: room temperature

> Safety Description:S22-24/25-37/39-26

> Danger Description:R36/37/38

> Danger Symbol: Xi

Synonyms|:

LABOTEST-BB LT00138111;1,4-PIPERAZINEBIS(ETHANESULFONIC ACID);1,4-PIPERAZINEDIETHANESULFONIC ACID;PIPERAZINE-1,4-BIS(2-ETHANESULFONIC ACID);PIPERAZINE-NN\'-BIS-2-ETHANESULPHONIC ACID;PIPERAZINE-N,N\'-BIS(2-ETHANESULFONIC ACID);PIPES;TIMTEC-BB SBB008973;PIPES, Free Acid, ULTROL Grade; 2,2-(piperazine-1,4-diyl)bis(ethanesulphonic) acid; PIPES Piperazine-N,N-bis(2-ethanesulfonic acid); PIPES, Molecular Biology Grade Piperazine-N,N-bis(2-ethanesulfonic acid), Molecular Biology Grade; PIPES, Free Acid, MB Grade (1.12030); 1,4-Piperazinebis(ethanesulfonic acid); Piperazine-1,4-bis(2-ethanesulfonic acid); Piperazine-N,N\'-bis(2-ethanesulfonic acid); Piperazine-1,4-bisethanesulfonic acid; LABOTEST-BB LT00138111; 1,4-PIPERAZINEDIETHANESULFONIC ACID; PIPERAZINE-NN\'-BIS-2-ETHANESULPHONIC ACID; TIMTEC-BB SBB008973; PIPES

Related categories:Biochemistry;Good\'s Buffers;Buffer

Related Products:68399-77-9;68189-43-5;10010-67-0;100037-69-2

The determination of UV absorbance

A certain amount of the sample was weighed to the nearest 0.0001g, into 10ml volumetric flask, after constant volume with pure water, at a specific wavelength by 2cm cuvette was measured by UV spectrophotometer.

Determination of melting point

Instrument:

Micro melting point apparatus WRS-2A, Melting point tubes

Determination of step:

Open switch, stable 20min.

First set the starting temperature than the melting point of the low 10 ℃, heating rate 2 ℃ / min.

The sample was grind as fine as possible and load in a clean, dry melting point pipe ,until the sample was compressed into about 1cm high .When the actual temperature reaches the set temperature and stabilized . Inserted into the capillary with the sample, the simultaneous determination of three.

The determination of solubility

Weigh a certain amount of samples into test tube, add a certain amount of water, shake and dissolve, observe the solution.

Detection of heavy metals

The meaning of heavy metals of this act is under the specified experimental condition, which can coloration effects with sodium sulfide or thioacetamide .The glasses of preparation or storage is not allowed to contain lead.

5.8.1 Reagents

a) Lead dinitrate:1μg/mL

b) Sodium hydroxide :10%(m/v)

c) Sodium sulfide :10%(m/v)

5.8.2 Preparation of lead standard solution

Weigh lead dinitrate 0.1599g ,relocated to 1000mL volumetric flask, plus nitric acid 5mL and water 50mL, diluted with water to the mark , shake, as the stock solution.

5.8.3 Determination of step

Before use, the exact amount of stock solution 1mL, relocated to a 100ml volumetric flask, dilute with water to the mark, shake, limit the use of the day .

Weigh sample 1g, after adding sodium hydroxide solution 5mL and 20mL of water to dissolve, set Nessler colorimetric tube, add 5 drops of sodium sulfide , shake , compared the color with 5mL lead standard solution after the same treatment, not deeper.

The determination of Loss on drying

Weigh a sample of approximately 2g (weighing accuracy 0.0001g),Record the weight of m,The sample was placed in a Petri dish constant weight (weight recorded as m0), the tile, the thickness of not more than 5mm, into the oven (As next dish cover removed) drying at 105-110 ℃ 3 hours.Whe