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Certification:ISO9001:2008

CAS NO 27565-41-9 ELISA Reagents DL-Dithiothreitol 99%

Quick Detail:

Name	DL-Dithiothreitol	Appearance	White  powder
Assay	≥99.0	Solubility(50mg/ml aq.)	Clear, colorless solution
Heavy Metals, ppm	≤5	PH(0.1M aq.)	4.0-6.5

Description:

> Formula: C4H10O2S2

> Weight:154.25

> Appearance:White  powder

> MP/℃:41.0-44.0

>Storage:2-8℃

> Safety Description:S26 S37/39

> Danger Description:R22 R36/37/38

> Danger Symbol:Xn

1.Applications:

  A. DTT acts as a cell permeable protective agent for Thiol groups..

  B. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE

  C.DTT is used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.

2.Location

  A. biology/biochemistry laboratory, pharmaceutical factory, cosmetics factory

3. Note:

  DTT is less pungent and is less toxic than 2-mercaptoethanol. It has been reported that a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol.

Specifications

Assay	≥99.0
MP/℃	41.0-44.0
Ultraviolet absorbance(0.1M aq.)	260nm:≤0.4 ;280nm:≤0.1
Heavy Metals, ppm	≤5
PH(0.1M aq.)	4.0-6.5
Shelf life	one year

Synonyms:

DTT-MBG;DTT (RACEMIC);DL-THREO-2,3-DIHYDROXY-1,4-DITHIOLBUTANE;DL-THREO-1,4-DIMERCAPTO-2,3-BUTANEDIOL;DL-DITHIOTHREITOL;DL-DTT;DITHIOTHREITOL, 1,4-;DITT;Clelands REDUCTACRYL; threo-1,4-Dimercapto-2,3-butanediol; 1,4-dithio-dl-threitol sol.; 1,4-Dithio-DL-threitol; dithiothreitol (dtt) sequencing grade; dithiothreitol molecular biology*reagent; DithiothreitollelandsReagenz; Dithithreitol; DL-Dithothreitol; (2S,3S)-1,4-disulfanylbutane-2,3-diol; DTT; (R,R)-Dithiothreitol; ; D-threo-1,4-Dimercapto-2,3-butanediol; 2,3-Butanediol, 1,4-dimercapto-, D-threo-; Cleland reagent; D-1,4-Dithiothreitol; D-Dtt; Sputolysin; Threitol, 1,4-dithio-; (R*,R*)-1,4-Dimercapto-2,3-butanediol; 1,4-Dithio-l-threitol; Cleland\'s reagent; Cleland regent; Clelands reagent; L-DTT; DL-1,4-Dithiothreitol; TPTZ; 2,4,6-Tri(2-pyridyl)-1,3,5-triazine

Related categories:

Biochemistry;O-Substituted Sugars;Sugar Alcohols;Sugars;Reagents for Electrophoresis

Related Products:

The determination of PH

Weigh a certain amount of samples into test tube, add a certain amount of water, shake and dissolve, determination of the pH used have been calibrated pH meter.

The determination of solubility

Weigh a certain amount of samples into test tube, add a certain amount of water, shake and dissolve, observe the solution.

Determination of melting point

   Instrument:

   Micro melting point apparatus WRS-2A, Melting point tubes

  Determination of step:

Open switch, stable 20min.

First set the starting temperature than the melting point of the low 10 ℃, heating rate 2 ℃ / min.

The sample was grind as fine as possible and load in a clean, dry melting point pipe ,until the sample was compressed into about 1cm high .When the actual temperature reaches the set temperature and stabilized . Inserted into the capillary with the sample, the simultaneous determination of three.

Detection of heavy metals

     The meaning of heavy metals of this act is under the specified experimental condition, which can coloration effects with sodium sulfide or thioacetamide  .The glasses of preparation or storage is not allowed to contain lead.

5.8.1  Reagents

  a) Lead dinitrate:1μg/mL

   b) Sodium hydroxide :10%(m/v)

   c) Sodium sulfide :10%(m/v)

5.8.2  Preparation of lead standard solution

     Weigh lead dinitrate  0.1599g ,relocated to 1000mL volumetric flask, plus nitric acid 5mL and water 50mL, diluted with water to the mark , shake, as the stock solution.

5.8.3 Determination of step

   Before use, the exact amount of stock solution 1mL, relocated to a 100ml volu